Effect of intracellular c-di-GMP level on AHL signal production. In trans expression of the c-di-GMP synthases, WspR from P. aeruginosa or the GGDEF domain of RpfR, in wild type H111 led to decreased AHL signal production; while overexpression of the c-di-GMP phosphodiesterases, RocR from P. aeruginosa or the EAL domain of RpfR resulted in increased AHL signal biosynthesis in BDSF-minus mutant ∆rpfFBc. Quantification of AHL signal production was performed with the aid of AHL reporter strain CF11. For convenient comparison, the AHL signal production of wild-type strain was defined as 100% and used to normalize the AHL signal production of other strains. The data presented are the means of three replicates and error bars represents the standard deviation.
Deng et al. BMC Microbiology 2013 13:148 doi:10.1186/1471-2180-13-148