Table 3

PFGE and fAFLP typing results from a panel of 97 L. monocytogenes isolates with index of discrimination (ID)
L. monocytogeneslineages Serogroups1or serotype2 Number of isolates Number of PFGE3types PFGE ID4 Number of fAFLP3types fAFLP ID4
I IVb 35 36 0.988 33 0.981
IIb 11
II IIa 45 45 0.995 43 0.989
IIc 5
III 4a 1 1 n/a 1 n/a
Total: 97 82 0.996 76 0.993

1 Serogrouping performed by multiplex PCR [4]: results are from both the European Reference Laboratory (EURL) for L. monocytogenes and the UK National Reference laboratory (UK-NRL) for Listeria.

2 Based on sero-agglutination performed by EURL.

3ApaI/AscI-PFGE was performed by the EURL and HindIII/HhaI-fAFLP by UK-NRL.

4 Index of discrimination calculated according to the Simpson’s index of diversity (ID) [25]. n/a: not applicable.

Roussel et al.

Roussel et al. BMC Microbiology 2013 13:14   doi:10.1186/1471-2180-13-14

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