Figure 3.

The recruitment of RhoA and Rac1 GTPases into parasitophorous vacuole membrane (PVM) is dependent on the GTPase activity (1000×). (A) The CFP-tagged dominant negative mutants RhoA N19 and Rac1 N17 were overexpressed in COS-7 cells and 48 hr post-transfection, the cells were infected with T. gondii RH tachyzoites. All of these mutant proteins did not accumulate on the PVM of T. gondii (arrowhead). (B) The CFP-tagged wild type RhoA and Rac1 were overexpressed in COS-7 cells and 48 hr post-transfection, the cells were infected with T. gondii RH tachyzoites. All of these wild-type proteins accumulated on the PVM of T. gondii (arrowhead). Bars: 10 μm.

Na et al. BMC Microbiology 2013 13:125   doi:10.1186/1471-2180-13-125
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