Confirmation of gene expression differences by RT-qPCR between day 2 spherules vs mycelia, day 8 spherules vs mycelia and day 8 vs day 2 spherules. The figure shows a comparison between the fold change for each gene for RT-qPCR data (grey bars) and microarray data (black bars) between the different conditions. RT-qPCR gene expression data (2-∆∆CT) was averaged within conditions and then used to calculate log2 fold change values between the different conditions for direct comparison to microarray data. A positive fold change indicates the gene was expressed to a greater extent within a condition. An asterisk (*) indicates that the gene was significantly differentially expressed (p <0.05, t-test) and the error bars on the RT-qPCR data represent the standard deviation between the biological replicates of mycelia, spherules at day 2 and spherules at day 8.
Viriyakosol et al. BMC Microbiology 2013 13:121 doi:10.1186/1471-2180-13-121