A heatmap depicting expression levels of genes in mycelia, day 2 spherules and day 8 spherules for the top 100 genes that were differentially expressed between mycelia, day 2 spherules and day 8 spherules. Fold changes were calculated for day 2 spherules vs mycelia and day 8 spherules vs mycelia. For each gene, the absolute peak log 2 fold change (FC) was identified across the three conditions and the raw expression values for the top 100 were log transformed and median-centered and included in the heatmap. Hierarchical clustering of genes and array samples based on their expression profiles is reflected in the dendrograms to the left and the top of the heatmap respectively and was performed by calculating distances using the Pearson correlation metric and then clustering distances using the average linkage method. The expression of genes marked with an asterisk (*) was confirmed by RT-qPCR. The scale is shown: red shading indicates greater expression blue shading represents lesser expression.
Viriyakosol et al. BMC Microbiology 2013 13:121 doi:10.1186/1471-2180-13-121