Open Access Highly Accessed Research article

Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease

Lotta Nylund12*, Reetta Satokari12, Janne Nikkilä23, Mirjana Rajilić-Stojanović45, Marko Kalliomäki6, Erika Isolauri6, Seppo Salminen1 and Willem M de Vos24

Author Affiliations

1 Functional Foods Forum, University of Turku, Turku, FI-20014, Finland

2 Department of Veterinary Biosciences and Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland

3 Finnish Red Cross Blood Service, Helsinki, Finland

4 Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands

5 Department for Biotechnology and Biochemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

6 Department of Pediatrics, University of Turku and Turku University Hospital, Turku, Finland

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BMC Microbiology 2013, 13:12  doi:10.1186/1471-2180-13-12

Published: 23 January 2013

Additional files

Additional file 1:

Basic characteristics of the study subjects.

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Additional file 2:

Primers targeting Bifidobacterium genus and species used in this study.

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Additional file 3:

Differences in bifidobacterial composition of all children at 6 and 18 months of age as assessed by using quantitative PCR.

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Additional file 4:

The histograms showing the distribution of p-values obtained from the statistical analyses of species-like level of HITChip data at 18 months. Each bar represents how many species-like groups gave a p-value in the given range when the effect of different factors on microbiota composition were analysed.

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Additional file 5:

The microbiota differences of healthy and eczematous children from placebo group as assessed by HITChip analysis.

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Additional file 6:

Bifidobacterial sub-communities in infants with eczema and healthy controls as assessed by quantitative PCR and HITChip analyses.

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Additional file 7:

Phylum-like (level 1) and genus-like (level 2) HITChip data used in this study. Data is presented as log-transformed values. A letter A refers to 6 months samples and a letter D to 18 months samples, respectively.

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Additional file 8:

P-values obtained from the statistical analysis of phylum-like and genus-like groups of HITChip data at 18 months. P-values are not corrected and therefore indicate trend-like differences in the abundance of individual bacterial groups between the groups of infants. Microbial groups that were over the detection level were included in the analysis.

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Additional file 9:

The microbiota differences between the intervention groups (LGG or placebo) at the age of 18 months as assessed by HITChip analysis.

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