Research article
Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease
1 Functional Foods Forum, University of Turku, Turku, FI-20014, Finland
2 Department of Veterinary Biosciences and Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland
3 Finnish Red Cross Blood Service, Helsinki, Finland
4 Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
5 Department for Biotechnology and Biochemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
6 Department of Pediatrics, University of Turku and Turku University Hospital, Turku, Finland
BMC Microbiology 2013, 13:12 doi:10.1186/1471-2180-13-12
Published: 23 January 2013Additional files
Additional file 1:
Basic characteristics of the study subjects.
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Additional file 2:
Primers targeting Bifidobacterium genus and species used in this study.
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Additional file 3:
Differences in bifidobacterial composition of all children at 6 and 18 months of age as assessed by using quantitative PCR.
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Additional file 4:
The histograms showing the distribution of p-values obtained from the statistical analyses of species-like level of HITChip data at 18 months. Each bar represents how many species-like groups gave a p-value in the given range when the effect of different factors on microbiota composition were analysed.
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Additional file 5:
The microbiota differences of healthy and eczematous children from placebo group as assessed by HITChip analysis.
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Additional file 6:
Bifidobacterial sub-communities in infants with eczema and healthy controls as assessed by quantitative PCR and HITChip analyses.
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Additional file 7:
Phylum-like (level 1) and genus-like (level 2) HITChip data used in this study. Data is presented as log-transformed values. A letter A refers to 6 months samples and a letter D to 18 months samples, respectively.
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Additional file 8:
P-values obtained from the statistical analysis of phylum-like and genus-like groups of HITChip data at 18 months. P-values are not corrected and therefore indicate trend-like differences in the abundance of individual bacterial groups between the groups of infants. Microbial groups that were over the detection level were included in the analysis.
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Additional file 9:
The microbiota differences between the intervention groups (LGG or placebo) at the age of 18 months as assessed by HITChip analysis.
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