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Open Access Highly Accessed Research article

Baseline survey of the anatomical microbial ecology of an important food plant: Solanum lycopersicum (tomato)

Andrea R Ottesen1*, Antonio González Peña2, James R White3, James B Pettengill1, Cong Li1, Sarah Allard1, Steven Rideout4, Marc Allard1, Thomas Hill1, Peter Evans1, Errol Strain1, Steven Musser1, Rob Knight2 and Eric Brown1

Author Affiliations

1 FDA Center for Food Safety and Applied Nutrition, Division of Microbiology, Molecular Methods and Subtyping, 5100 Paint Branch Parkway, College Park, MD 20740, USA

2 Department of Chemistry and Biochemistry, University of Colorado at Boulder 215 UC, Boulder, CO 80309-0215, USA

3 IGS Institute for Genome Sciences University of Maryland School of Medicine, 801 West Baltimore St., Baltimore, MD 21201, USA

4 Virginia Polytechnic Institute and State University Eastern Shore AREC, 33446 Research Drive, Painter, VA 23420, USA

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BMC Microbiology 2013, 13:114  doi:10.1186/1471-2180-13-114

Published: 24 May 2013

Abstract

Background

Research to understand and control microbiological risks associated with the consumption of fresh fruits and vegetables has examined many environments in the farm to fork continuum. An important data gap however, that remains poorly studied is the baseline description of microflora that may be associated with plant anatomy either endemically or in response to environmental pressures. Specific anatomical niches of plants may contribute to persistence of human pathogens in agricultural environments in ways we have yet to describe. Tomatoes have been implicated in outbreaks of Salmonella at least 17 times during the years spanning 1990 to 2010. Our research seeks to provide a baseline description of the tomato microbiome and possibly identify whether or not there is something distinctive about tomatoes or their growing ecology that contributes to persistence of Salmonella in this important food crop.

Results

DNA was recovered from washes of epiphytic surfaces of tomato anatomical organs; leaves, stems, roots, flowers and fruits of Solanum lycopersicum (BHN602), grown at a site in close proximity to commercial farms previously implicated in tomato-Salmonella outbreaks. DNA was amplified for targeted 16S and 18S rRNA genes and sheared for shotgun metagenomic sequencing. Amplicons and metagenomes were used to describe “native” bacterial microflora for diverse anatomical parts of Virginia-grown tomatoes.

Conclusions

Distinct groupings of microbial communities were associated with different tomato plant organs and a gradient of compositional similarity could be correlated to the distance of a given plant part from the soil. Unique bacterial phylotypes (at 95% identity) were associated with fruits and flowers of tomato plants. These include Microvirga, Pseudomonas, Sphingomonas, Brachybacterium, Rhizobiales, Paracocccus, Chryseomonas and Microbacterium. The most frequently observed bacterial taxa across aerial plant regions were Pseudomonas and Xanthomonas. Dominant fungal taxa that could be identified to genus with 18S amplicons included Hypocrea, Aureobasidium and Cryptococcus. No definitive presence of Salmonella could be confirmed in any of the plant samples, although 16S sequences suggested that closely related genera were present on leaves, fruits and roots.

Keywords:
Tomato microflora; 16S; 18S; Metagenomics; Phyllosphere; Solanum lycopersicum; Tomato organs; Microbial ecology; Baseline microflora; Tomatome