Table 8

Details of MAP strains used in this study
Name Origin and source Medium used for maintenance and propagation
316FNOR 1960 (Vaccine strain) Obtained from the VLA in 1960 and used in a vaccine trial in goats in Norway during the 1960s [15]. Maintained at the Norwegian Veterinary Institute, Oslo. Selective Dubos medium [47] supplemented with mycobactin (2 μg/ml) and pyruvate (4 mg/ml)
316FCYP1966 (Vaccine strain) Obtained from the VLA in 1966 as lyophilised aliquots and used to vaccinate goats in Cyprus during the 1960s [18]. Strain used in this study was recovered from an aliquot lyophilised on 04 January 1966 and resuscitated in 2009 with limited passage since. 7H9*
316FNLD1978 (Vaccine strain) Obtained from the VLA in 1978 and used as a killed vaccine [38]. Maintained at the Central Veterinary Institute, Lelystad, Netherlands. Potato starch medium (P.Willemsen personal communication)
316FNEO4/81 (Vaccine strain) Neoparasec vaccine (Merial, France) subcultures from a stock [25] assumed to be derived from a 316 F Weybridge UK strain purchased in the 1980s. 7H9* or 7H11**
316FNEO8/81 (Vaccine strain)
316FNEO68451-2 (Vaccine strain)
316FNEO69341 (Vaccine strain)
316v Australian strain derived from a variant labelled 316f around 1986 [48] which itself was obtained from a New Zealand source who obtained the strain in the early 1980s. Maintained at the University of Sydney, Sydney, Australia. Mycobactin free medium but grew poorly (R Whittington, personal communication)
316FUK2000 (Vaccine strain) Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK, with limited passage. Liquid Watson Reid
316FUK2001 (Vaccine strain) Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. 7H11**
316FNLD2008 (Vaccine strain) Obtained from VLA in 2008 and maintained at the Central Veterinary Institute, Lelystad, Netherlands HEYM
IIUK2000 (Vaccine strain) Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK. Liquid Watson Reid[14]
IIUK2001 (Vaccine strain) Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. 7H11**
2eUK2000 (Vaccine strain) Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK. Liquid Watson Reid ‘A’ Block†† medium
2eUK2001 (Vaccine strain) Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. 7H11**
MAPK10 (Wild type strain) Purchased from ATCC: BAA-968. Sequenced reference strain isolated from a cow in 1990. 7H9* or 7H11**
CAM87 (Wild type strain) MAP Type III strain isolated from a goat in 2005 [26] and maintained at the Universidad Complutense de Madrid, Madrid, Spain. 7H9*
JD87/107 (Wild type strain) Isolated from a deer in 1987 and maintained at the Moredun Research Institute, Scotland, UK. 7H11**

*7H9: Middlebrooks 7H9 (Becton Dickinson, UK) supplemented with 2 mg/L Mycobactin J (Allied Monitor, USA), 0.5% glycerol, 10% oleic acid-albumin-dextrose-catalase (OADC) enrichment medium (Difco, UK), 25 mg/L amphotericin B, 35 mg/L naladixic acid and 35 mg/L vancomycin.

**7H11: Middlebrooks 7H11 (Becton Dickinson, UK) agar supplemented with 2 mg/L Mycobactin J (Allied Monitor, USA), 2.5% glycerol (v/v), 10% OADC (v/v) enrichment medium (Difco, UK), 20% (v/v) new born calf serum, 0.3 g/L asparagine, Mycobacteria Selectatabs (10 mg/L amphotericin B, 200,000 units/L polymixin B, 100 mg/L ticarcillin and 10 mg/L trimethoprim [MAST Laboratories Ltd, UK]).

Liquid Watson Reid: Asparagine 5 g/l, Potassium dihydrogen phosphate 2 g/l, Magnesium suphate 1 g/l, Tri-ammonium citrate 2 g/l, Sodium chloride 2 g/l, D(+) Glucose 10 g/l, Glycerol 48 ml/l, Ammonium iron (III) citrate brown 0.075 g/l, 1.33mls of Supplement A: 2 g/l Zinc sulphate, 2 g/l Copper sulphate, 1 g/l Cobalt nitrate, 1.33mls of Supplement B; 50 g/l Calcium chloride.

††Liquid Watson Reid ‘A’ Block: as Watson Reid medium but without supplements A and B.

Bull et al.

Bull et al. BMC Microbiology 2013 13:11   doi:10.1186/1471-2180-13-11

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