Table 1

Primers used for cytotoxin-associated gene ( cagA ) and vacuolating cytotoxin gene ( vacA ) typing of H. pylori
Gene target Primer designation Nucleotide sequence Amplicon size (bp)
vacAS-F VacAS-F 5’-ATGGAAATACAACAAACACAC-3’ 259 (type s1)
VacAS-R 5’-CTGCTTGAATGCGCCAAAC-3’ 286 (type s2)
vacA midregion VacAM-F 5’-CAATCTGTCCAATCAAGCGAG-3’ 567 (type m1)
VacAM-R 5’-GCGTCAAAATAATTCCAAGG-3’ 642 (type m2)
cagA CagA-F 5’-GATAACAGGCAAGCTTTTGAGAGGGA-3’ 393
CagA-R 5’-CCATGAATTTTTGATCCGTTCGG-3’

Bisignano et al.

Bisignano et al. BMC Microbiology 2013 13:103   doi:10.1186/1471-2180-13-103

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