Cytokine production by the draining lymph nodes after different inoculums of SGE. BALB/c mice inoculated i.d. once (SGE-1X ) or three times (SGE-3X) with Lutzomyia longipalpis SGE or with PBS (control) were challenged with 105L. braziliensis stationary phase promastigote forms. At the end of the 7th week post-infection, draining lymph node cells were harvested and restimulated in vitro with L. braziliensis antigen (5 μg/ml) or medium for 72 h. IL-10 (A) and IFN-γ (B) levels in the supernatant were determined by ELISA assay. The results are expressed as the mean ± SEM of at least two independent experiments using four to five mice per group (n = 4-5 mice per group). #P < 0.05 compared with medium-only stimulus. *P < 0.05 compared with the SGE-1X group.
Carregaro et al. BMC Microbiology 2013 13:102 doi:10.1186/1471-2180-13-102