This article is part of the supplement: Arthropod symbioses: from fundamental studies to pest and disease management

Open Access Research

Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina)

Vangelis Doudoumis1,8, George Tsiamis1,8, Florence Wamwiri2,5, Corey Brelsfoard2,3, Uzma Alam2, Emre Aksoy2, Stelios Dalaperas1,8, Adly Abd-Alla4, Johnson Ouma5, Peter Takac6, Serap Aksoy2 and Kostas Bourtzis1,7,8*

1 Department of Environmental and Natural Resources Management, University of Ioannina, 2 Seferi St, 30100 Agrinio, Greece

2 Yale University School of Public Health, 60 College St., 811 LEPH, New Haven, CT 06520, USA

3 Current address: Department of Entomology, University of Kentucky, S-225 Ag. Science Center North, Lexington, KY 40546, USA

4 Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria

5 Trypanosomiasis Research Centre, Kenya Agricultural Research Institute, P.O. Box 362, Kikuyu 00902, Kenya

6 Institute of Zoology, Section of Molecular and Applied Zoology, Slovak Academy of Science, Dubravska cesta 9, 845 06 Bratislava, Slovakia

7 Biomedical Sciences Research Center Al. Fleming, 16672 Vari, Greece

8 Present Address: Department of Environmental and Natural Resources Management, University of Western Greece, 2 Seferi St, 30100 Agrinio, Greece

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BMC Microbiology 2012, 12(Suppl 1):S3 doi:10.1186/1471-2180-12-S1-S3

Published: 18 January 2012

Additional files

Additional file 1:

Supplementary Table 1: Primers used in the present study.

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Additional file 2:

Supplementary Figure 1: Maximum likelihood inference phylogeny based on the concatenated MLST data, 2,079 bp. (Please note that tree has been rooted to the supergroup D sequences).

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Additional file 3:

Supplementary Figure 2: Maximum likelihood inference phylogeny based on the on the wsp sequence. (Please note that tree has been rooted to the supergroup D sequences).

Format: JPG Size: 124KB Download file

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