Open Access Research article

Sequence analysis for detection of first-line drug resistance in Mycobacterium tuberculosis strains from a high-incidence setting

Silke Feuerriegel1*, Barbara Oberhauser2, Abu Garawani George3, Foday Dafae4, Elvira Richter5, Sabine Rüsch-Gerdes5 and Stefan Niemann1

Author Affiliations

1 Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany

2 German Leprosy and TB Relief Association, Würzburg, Germany

3 National Leprosy/TB Reference Laboratory, Freetown, Sierra Leone

4 Manager of the National Leprosy and Tuberculosis Programme (NLTP), Ministry of Health and Sanitation, Freetown, Sierra Leone

5 National Reference Center for Mycobacteria, Research Center Borstel, Borstel, Germany

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BMC Microbiology 2012, 12:90  doi:10.1186/1471-2180-12-90

Published: 30 May 2012

Additional files

Additional file 1:

PCR primers and conditions used for amplification and sequencing. In this table a summary of all primers, including oligonucleotide sequences, used in this study for both DNA amplification and sequencing is given.

Format: DOCX Size: 18KB Download file

Open Data

Additional file 2:

Summary of all sequencing, DST, MIC and genotyping data. This table summarizes all data generated in this study. It comprises sequencing, DST (drug susceptibility testing) and MIC (minimal inhibitory concentration) testing results as well as all genotyping data.

Format: XLSX Size: 54KB Download file

Open Data