Table 1

Strains and Plasmids
Strains/Plasmids Characteristics Reference
Strains
P. aeruginosa
PAO1 ATCC15692 [29]
KG4509 ΔmexB derivative of PAO1 This study
KG7004 ΔlasI ΔrhlI derivative of PAO1 This study
KG7050 ΔlasI ΔrhlIΔmexB derivative of PAO1 This study
KG7403 gfp fused to the lasB promoter and integrated at the attB site of the KG7004 chromosome This study
KG7503 gfp fused to the lasB promoter and integrated at the attB site of the KG7050 chromosome This study
E. coli
DH5α F-, Φ80d lacZ ΔM15, Δ(lacZYA- argF')U169, deoR, recA1, endA1, hsdR17(rk- mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 [30]
S17-1 RE42-Tc: Mu-Km:: Tn7 pro res mod4 [31]
Plasmids
pUC18 Apr; high-copy-number cloning vector [32]
pBR322 Apr Tcr; high-copy-number cloning vector [33]
pSL1180 super-polylinker phagemid [34]
pTO003 Gmr; E. coli-P. aeruginosa shuttle expression vector [35]
pMT5059 Cbr; pBend2 derivative carrying multiple-cloning site and Not I site [36]
pMT5071 Cmr; pMOB3 derivative carrying Ω-Cm instead of Cm [37]
pAF2071 Cbr Cmr; pKT5059 carrying 2911-bp fragment with 3′ flanking region of rhlI including 91-bp of rhlI and 2110-bp fragment with 5′ flanking region of rhlI Mob cassette from pMT5071 at Not I This study
plasI Cbr Cmr; pMT5059 carrying 1.0-kb PCR fragments with 3′ and 5′ flanking regions of lasI and Mob cassette from pMT5071 at Not I This study
pMexB Cbr Cmr; pMT5059 carrying 1.0-kb PCR fragments with 3′ and 5′ flanking regions of mexB and Mob cassette from pMT5071 at Not I This study
pKTA113 Gmr; subcloning of full length mexB in Xba I and Hin dIII site of pTO003 This study
pYT57 Phe136Ala mutation of mexB on pKTA113 This study
pYT81 Asp681Ala mutation of mexB on pKTA113 This study
pGreen Apr; Enhanced GFP cassette plasmid [38]
mini-CTX1 Tcr; self-profcient integration vector with tet, V-FRT-attP-MCS, ori, int, and oriT [39]
pSG Tcr; subcloning of gfp from pGreen This study
pSQG003 Tcr; subcloning of a 598-bp lasB promoter region between Hin dIII and Kpn I sites of pSG This study
pFLP2 Apr; source of Flp recombinase [40]
pME6012 Tcr; pVS1-p15A shuttle vector [41]
pMQG003 Tcr; subcloning of a 1781-bp lasB promoter::gfp region from pSQG003 in Bgl II site of pME6012 This study

Minagawa et al.

Minagawa et al. BMC Microbiology 2012 12:70   doi:10.1186/1471-2180-12-70

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