Role of MexAB-OprM in cross-talk betweenP. aeruginosaandV. anguilarum. The two monitor strains, KG7004 (ΔlasI ΔrhlI) and KG7050 (ΔlasI ΔrhlIΔmexB) harboring the lasB promoter-gfp plasmid (pMQG003) were used. P. aeruginosa PAO1(a, b, c and d) or V. anguilarum (e, f, g and h) and P. aeruginosa KG7004 (bottom), were cross-streaked on a LB agar plate against a monitor strain (center). Following 24 h incubation at 30°C, growth of the strains was observed under a stereomicroscope (a, c, e and g), and then production of GFP by the monitor strains was visualized by excitation of the plates with blue light (b, d, f and h).
Minagawa et al. BMC Microbiology 2012 12:70 doi:10.1186/1471-2180-12-70