3-oxo-Cn-HSLs are selected by MexAB-OprM inP. aeruginosa. Individual cultures of KG7403 (ΔlasI ΔrhlI PlasB-gfp) and KG7503 (ΔlasI ΔrhlIΔmexB PlasB-gfp) were grown in LB medium with 5 μM 3-oxo-C9-HSL (a), 3-oxo-C10-HSL (b), or 3-oxo-C12-HSL (c), respectively. Transcription of lasB was determined by measurement of the fluorescence intensity (arbitrary units) depending on the amount of green-fluorescence protein (GFP) derived from PlasB-gfp; emission at 490 nm and excitation at 510 nm. MexAB-OprM efflux activity was inhibited by 0, 0.05 or 0.5 μM ABI. Open bars, KG7403; closed bars, KG7503. The data represent mean values of three independent experiments. Error bars represent the standard errors of the means.
Minagawa et al. BMC Microbiology 2012 12:70 doi:10.1186/1471-2180-12-70