Neutralization by PCT of LPS from S. typhimurium and E. coli. The effect of PCT on S. typhimurium and E. coli LPS (100 pg/ml) reactivity was evaluated as O. D. (405 nm) by the chromogenic LAL test after 30 minutes incubation of the above reported LPS concentration: with 0 pg/ml PCT (LPS 30 min), with 5000 pg/ml PCT (LPS + PCT 5000 30 min), 500 pg/ml PCT (LPS + PCT 500 30 min). Results are presented as means ± SEM of at least four experiments each carried out in duplicate. Statistical significance between groups was assessed by Student’s t test. A p < 0.05 was considered significant, whereas not significant (n.s.) difference was associated with a p ≥ 0.05. Statistics were performed in comparison with respective LPS type-stimulated PCT-untreated cells (LPS 30 min), and the exact significance index is indicated on the top of the horizontal line encompassing the two statistically compared bars.
Matera et al. BMC Microbiology 2012 12:68 doi:10.1186/1471-2180-12-68