Open Access Research article

Multilocus genetics to reconstruct aeromonad evolution

Frédéric Roger1, Hélène Marchandin12, Estelle Jumas-Bilak13, Angeli Kodjo45, the colBVH study group6 and Brigitte Lamy1256*

Author Affiliations

1 Laboratoire de Bactériologie-Virologie (UMR 5119 - Equipe Pathogènes et Environnements), Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France

2 Laboratoire de Bactériologie, Centre Hospitalier Régional Universitaire de Montpellier (Hôpital Arnaud de Villeneuve), 371, Avenue du Doyen Gaston Giraud, 34295, Montpellier Cedex 5, France

3 Laboratoire d’Hygiène hospitalière, Centre Hospitalier Universitaire de Montpellier, 778, Rue de la Croix Verte, 34000, Montpellier, France

4 CNRS UMR 5557 Ecologie microbienne, VetAgro Sup Campus vétérinaire de Lyon, 69280, Marcy-l’Étoile, France

5 Groupe d’ Etude Français des Aeromonas (GFA), Lyon, France

6 ColBVH, Collège de bactériologie, virologie et hygiène des hôpitaux généraux, Lyon, France

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BMC Microbiology 2012, 12:62  doi:10.1186/1471-2180-12-62

Published: 30 April 2012

Additional files

Additional file 1:

Figure S1. Unrooted maximum-likelihood tree based on concatenated sequences of five housekeeping gene fragments (gltA, gyrB, rpoB, tsf, zipA, 2724 nt). The horizontal lines indicate genetic distance, with the scale bar indicating the number of substitutions per nucleotide position. The numbers at the nodes are support values estimated with 100 bootstrap replicates. Only bootstrap values > 70 are shown on the tree. The clades defined in Table 1 are indicated with brackets at the top right of the figure. Only type strains and reference strains are represented in the tree.

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Additional file 2:

Table S2. Recombination event types and recombinant sequences.

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Additional file 3:

Figure S3. SplitsTree decomposition analyses of the MLSA data for strains belonging to theA. caviae(a), A. hydrophila(b) andA. veronii(c) clades. The distance matrix was obtained from the allelic profiles of the sequence types (ST). A network-like graph indicates recombination events. Star-like radiation from the central point indicates an absence of recombination. The names of eBURST clonal complexes (CCs), as defined in the text and in Table 1, are indicated near the corresponding STs. The number of strains sharing an identical ST is indicated below the ST number in brackets. Type strain STs are indicated by dots.

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