Table 4

Laboratory quantitative validation results of the BactQuant assay performed using pure plasmid standards and different mixed templates
Templates used Assay dynamic range Average reaction efficiency (SD) r2–value
Plasmid standards–only (10 μl Rxn) 100–108 copies 102% (2%) >0.999
Plasmid standards-only (5 μl Rxn) 100 – 108 copies 95% (1%) >0.999
Plasmid standardsplus0.5 ng human gDNA 100 – 108 copies 99% (4%) >0.994
Plasmid standardsplus1 ng human gDNA 100 – 108 copies 101% (5%) >0.994
Plasmid standardsplus5 ng human gDNA 500 – 108 copies 96% (1%) >0.999
Plasmid standardsplus10 ng human gDNA 1000 – 108 copies 97% (2%) >0.999
Plasmid standardsplus0.5 ngC. albicansgDNA 100 – 108 copies 97% (1%) >0.999

Liu et al.

Liu et al. BMC Microbiology 2012 12:56   doi:10.1186/1471-2180-12-56

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