A-B. Standard curve amplification profiles of the BactQuant assay generated from 10 μl and 5 μl reactions using seven ten-fold dilutions and normalized plasmid standards at 109copies/μl. The Ct value of standard curve using 5 μl reaction volumes (Figure2B) shows an approximately 1 Ct left shift from the 10 μl reaction volumes (Figure2A). However, the overall amplification profiles are not significantly different between the different reaction volumes over the assay dynamic range of 102 copies to 108 copies of 16 S rRNA gene per reaction.
Liu et al. BMC Microbiology 2012 12:56 doi:10.1186/1471-2180-12-56