Resolution:
## Figure 1.
Results fromin silicocoverage analysis of the BactQuant assay using the stringent criterion against 1,849
genera and 34 phyla showing broad coverage. The number of covered genus for each phylum analyzed ( left) and the list of all uncovered genera ( right) are shown. On the circular 16 S rRNA gene-based maximum parsimony phylogeny ( left), each of the covered ( in black) and uncovered ( in red) phylum by the BactQuant assay is annotated with the genus-level numerical coverage
in parenthesis below the phylum name. Each genus-level numerical coverage annotation
consists of a numerator (i.e., the number of covered genus for the phylum), a denominator
(i.e., the total number of genera eligible for sequence matching for the phylum),
and a percentage calculated using the numerator and denominator values. Comparison
with the published assay is presented for each phylum as notations of a single asterisk
(*) for phylum not covered by the published assay and as a double asterisk (**) for
phylum with <50% of its genera covered by the published qPCR assay. The phylum and
genus taxonomic identifications of all genera not covered by the BactQuant assay are
also presented ( right) (Unc = Unclassified).
Liu |