Table 4

Cross-correlations between experimental and standard digital T-RFLP profiles
Samples Optimal cross-correlation lag between digital and experimental T-RFLP profilesa(bp) Maximum cross-correlation coefficient at optimal lagb(−) Total number of experimental T-RFs per profile (−) Number of experimental T-RFs affiliated with digital T-RFsc(−) Percentage of experimental T-RFs affiliated with digital T-RFsc(%)
Groundwater
GRW01d −4 0.62 88 58 66
GRW02d −5 0.69 50 23 46
GRW03d −4 0.44 76 62 82
GRW04d −5 0.71 44 24 44
GRW05d −5 0.35 75 56 75
GRW06d −6 0.51 87 70 81
Avg±stdev (min-max) −5±1 0.55±0.14 70±19 49±20 67±14
-(4–6) (0.35-0.71) (44–88) (23–70) (44–82)
GRW07e −6 0.70 57 17 30
GRW08e −4 0.59 54 43 80
GRW09e −4 0.69 71 66 93
GRW10e −5 0.68 70 22 31
Avg±stdev (min-max) −5±1 0.67±0.05 59±11 34±20 59±33
-(4–6) (0.59-0.70) (44–71) (17–66) (30–93)
Aerobic granular sludge
AGS01e −5 0.75 48 31 65
AGS02e,f −5 0.90 38 22 58
AGS03e,f −5 0.90 38 19 50
AGS04e −5 0.72 52 24 46
AGS05e −4 0.67 43 29 67
AGS06e,f −5 0.91 38 19 50
AGS07e −5 0.80 38 31 82
Avg±stdev (min-max) −5±0 0.82±0.10 42±6 25±5 61±12
-(4–5) (0.67-0.91) (38–52) (19–31) (46–82)

a Shift leading to optimal matching of the digital to the experimental T-RFLP profile.

b Maximum cross-correlation coefficients obtained after matching of the digital to the experimental T-RFLP profile.

c Number and percentage of experimental T-RFs having corresponding digital T-RFs.

d Samples GRW01-06 were pyrosequenced with the HighRA method.

e Samples GRW07-10 and AGS01-07 were pyrosequenced with the LowRA method.

f Samples AGS02, AGS03, and AGS06 are triplicates from the same DNA extract.

Weissbrodt et al.

Weissbrodt et al. BMC Microbiology 2012 12:306   doi:10.1186/1471-2180-12-306

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