VIDISCR method for virus identification. (A) Schematic overview of steps in VIDISCR method. (B) Examples of VIDISCR-mediated virus identification. Specimens were analyzed using ethidium bromide-stained agarose gels (SV5 and SV40). Lane M, DNA molecular weight markers (DL2000,TOKARA); –, negative controls; +, VIDISCR PCR products for SV5 SV40 (amplified with primer S15, S14 , respectively). (C) VIDISCR PCR products for YN08. S11 primer was used for selective amplification; products were visualized by EB-stained agarose gel electrophoresis. Lanes 1 and 2, duplicate control supernatant from uninfected Kunming strain suckling mice; 3 and 4, duplicate PCR product of cultured YN08 harvested from brain tissues of Kunming strain suckling mice; M, DNA molecular weight markers (DL2000, Takara). Arrow indicates YN08 fragment that was excised from gel and sequenced. (D) VIDISCR PCR products for YN08 amplified with different primers. Lanes 1–10, PCR product of cultured YN08 amplified with different primers S1, S12, S13, S15, S21, S22, S23, S25, S38, S40, respectively; M, DNA molecular weight markers (DL2000, Takara).
Hu et al. BMC Microbiology 2012 12:305 doi:10.1186/1471-2180-12-305