Research article
Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates
1 INRA, UR 1282 Infectiologie Animale et Santé Publique, Agents transmissibles et Infectiologie, F-37380, Nouzilly and IFR, 136, France
2 Université de Tours, UMR1282 Infectiologie et Santé Publique, F-37000, Tours, France
3 ANSES LERQAP, Unité Caractérisation et Epidémiologie Bactérienne, F-94706, Maisons-Alfort, France
4 Institut Pasteur, French National Reference Center and WHO collaborating Center for Listeria, F-75724, Paris, France
5 INRA, UR 0341 Mathématiques et Informatique Appliquées, F-78352, Jouy-en-Josas, France
6 PHYLOGENE, F-30620, Bernis, France
7 Université François Rabelais de Tours, EA3854 « Bactéries et risque materno-fœtal », Tours, France and CHRU, F-37044, Tours, France
8 Current address: ANSES UHQPAP, Unité Hygiène et Qualité des Produits Avicoles et Porcins, F-22440, Ploufragan, France
9 Current address: Université Paris Sud Orsay, CNRS-UMR807, Unité Ecologie Systématique Evolution, F-91400, Orsay, France
10 Current address: Institut Pasteur, Centre de Ressources Biologiques de l’Institut Pasteur (CRBIP), F-75724, Paris, France
11 Current address: Laboratoire de Microbiologie-Hygiène, Centre Hospitalier de Versailles, Université Paris Sud, F-78150, Le Chesnay, France
BMC Microbiology 2012, 12:304 doi:10.1186/1471-2180-12-304
Published: 26 December 2012Additional files
Additional file 1:
Describes theListeria strains used in this article[[7],[8],[10],[15],[26-30]].
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Additional file 2:
Describes the primers used for the amplification and sequencing of the housekeeping genesabcZ,bglA,dapE, dta, kat,ldh and lhkAand the virulence genes prfA, actAandinlA. The primers used for the verification of an inserted fragment in the “clpP” region have been also given.
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