Open Access Highly Accessed Research article

Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates

Sylvie M Roche12*, Olivier Grépinet12, Annaëlle Kerouanton38, Marie Ragon49, Alexandre Leclercq4, Stéphanie Témoin1102, Brigitte Schaeffer5, Gilbert Skorski6, Laurent Mereghetti27, Alban Le Monnier114 and Philippe Velge12

Author Affiliations

1 INRA, UR 1282 Infectiologie Animale et Santé Publique, Agents transmissibles et Infectiologie, F-37380, Nouzilly and IFR, 136, France

2 Université de Tours, UMR1282 Infectiologie et Santé Publique, F-37000, Tours, France

3 ANSES LERQAP, Unité Caractérisation et Epidémiologie Bactérienne, F-94706, Maisons-Alfort, France

4 Institut Pasteur, French National Reference Center and WHO collaborating Center for Listeria, F-75724, Paris, France

5 INRA, UR 0341 Mathématiques et Informatique Appliquées, F-78352, Jouy-en-Josas, France

6 PHYLOGENE, F-30620, Bernis, France

7 Université François Rabelais de Tours, EA3854 « Bactéries et risque materno-fœtal », Tours, France and CHRU, F-37044, Tours, France

8 Current address: ANSES UHQPAP, Unité Hygiène et Qualité des Produits Avicoles et Porcins, F-22440, Ploufragan, France

9 Current address: Université Paris Sud Orsay, CNRS-UMR807, Unité Ecologie Systématique Evolution, F-91400, Orsay, France

10 Current address: Institut Pasteur, Centre de Ressources Biologiques de l’Institut Pasteur (CRBIP), F-75724, Paris, France

11 Current address: Laboratoire de Microbiologie-Hygiène, Centre Hospitalier de Versailles, Université Paris Sud, F-78150, Le Chesnay, France

For all author emails, please log on.

BMC Microbiology 2012, 12:304  doi:10.1186/1471-2180-12-304

Published: 26 December 2012

Additional files

Additional file 1:

Describes theListeria strains used in this article[[7],[8],[10],[15],[26-30]].

Format: DOC Size: 108KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Describes the primers used for the amplification and sequencing of the housekeeping genesabcZ,bglA,dapE, dta, kat,ldh and lhkAand the virulence genes prfA, actAandinlA. The primers used for the verification of an inserted fragment in the “clpP” region have been also given.

Format: DOC Size: 55KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data