Table 2

Combined dataset of full list of proteins identified in pool B after direct trypsin digestion and SDS-PAGE separation
Accession number Full identification name Biological function known or inferred Localisation Mascot score Total matches Peptides matched Sequence coverage (%)
A1VXN0 (Cj0146c)* Thioredoxin reductase Oxidation-reduction process Cytoplasm 353 10 4 20
A1VXN1 (cj0147c) Thioredoxin Metabolic Cytoplasm 1931 41 4 56
A1VXS1 (Cj0193c) Trigger factor Cell division Cytoplasm 33 1 1 2
62c 4c 3c 6c
A1VXZ3 (Cj0269) Branched-chain amino acid aminotransferase Metabolic Cytoplasm 146 10 3 17
A1VY51 (Cj0332c) Nucleoside diphosphate kinase Nucleotide metabolism Cytoplasm 80 2 1 16
A1VY53 (Cj0334) Antioxidant, AhpC/Tsa family Cell redox homeostasis Cytoplasm 1762 62 6 41
90c 5c 5c 33c
A1VYF1 (Cj0434) 2,3-bisphosphoglycerate-independent phosphoglycerate Gluconeogenesis Cytoplasm 70 6 3 6
50c 4c 4c 9c
A1VYI6 (Cj0470) Elongation factor Tu (tuf) Protein biosynthesis Cytoplasm 94 7 3 10
53c 4c 3c 10c
A1VYJ5 (Cj0479) DNA-directed RNA polymerase subunit beta Transcription Cytoplasm 48 5 1 0
A1VYM1 (Cj0509c) ATP-dependent chaperone protein ClpB Metabolic Cytoplasm 110 6 2 3
100c 6c 5c 5c
A1VZ00 (Cj0640c) Aspartyl-tRNA synthetase (aspS) Protein biosynthesis Cytoplasm 44 3 2 6
A1VZH9 (Cj0835c) Aconitate hydratase Metabolic Cytoplasm 97 3 2 3
127c 7c 6c 11c
A1VZR2 (Cj0929) Cytosol aminopeptidase family protein Proteolysis Cytoplasm 68 3 1 2
39c 3c 3c 5c
A1W057 (Cj1070) 30S ribosomal protein S6 Translation Cytoplasm 226 8 1 8
A1W0G5 (Cj1181c) Elongation factor Ts (tsf) Protein biosynthesis Cytoplasm 201 5 2 10
A1W0K3 (Cj1220) 10 kDa chaperonin (groS) Protein folding Cytoplasm 689 37 4 47
A1W0K4 (Cj1221) 60 kDa chaperonin (groL) Protein folding Cytoplasm 505 27 7 19
179c 6c 5c 13c
A8FKV4 (Cj0531) Isocitrate dehydrogenase, NADP-dependent Metabolic Cytoplasm 38 7 3 6
355c 14c 12c 17c
A8FNK5 (Cj1545c) MdaB-like protein Oxidation-reduction process Cytoplasm 81 3 2 12
A1VZC7 (Cj0779) Thiol peroxidase Oxidation-reduction process Cytoplasm / periplasm 679 14 4 37
A8FKM7 (Cj0440c) Putative transcriptional regulator Transcription Cytoplasma 82 3 3 12
A1VXI8 (Cj0105) ATP synthase subunit alpha ATP synthesis Cytoplasmic membrane 84 3 2 9
A1W0Q1 (Cj1266c) Quinone-reactive Ni/Fe-hydrogenase, large subunit (hydB) Oxidation-reduction process Cytoplasmic membrane 200 7 3 9
A1W0V5 (cj1338c) Flagellin B Flagellar motility Extracellular 2023 48 8 23
608c 25c 13c 34c
A1VY12 (cj0289c) Major antigenic peptide PEB3 Transport Non-cytoplasmic 173 4 2 11
304c 8c 6c 27c
A1VZC6 (cj0778) Major antigenic peptide PEB2 Transport Non-cytoplasmic 233 9 3 27
159c 8c 6c 30c
A1VZK9 (Cj0866) Arylsulfate sulfotransferase, degenerate Transferase Non-cytoplasmic 358 10 4 8
A1VZY6 (cj0998c) Putative periplasmic protein Unknown Non-cytoplasmic 597 19 3 25
A1W0Q2(cj1240c) Putative periplasmic protein Protein binding Non-cytoplasmic 151 11 3 13
A8FKY2 (Cj0559) Putative pyridine nucleotide-disulphide oxidoreductase Oxidation-reduction process Non-cytoplasmic 200 7 3 9
292c 17c 13c 49c
A8FLP3 (cj0834c) Putative uncharacterised protein Protein-protein interaction Non-cytoplasmic a 155c 8c 7c 19c
A8FKK7 (Cj0420) Putative uncharacterised protein Lipid binding protein Non-cytoplasmic, likely periplasmic 331 12 2 14
A1VYD4 (Cj0414) Putative oxidoreductase subunit Oxidation-reduction process Non-cytoplasmicb 581 11 3 26
A1VXJ7 (cj0114) Putative periplasmic protein Protein binding Outer membrane 174 5 1 4
A1VXA8 (Cj0029)* L-asparaginase Metabolic Periplasm 139 3 1 4
63c 3c 3c 14c
A1VXB4 Gamma-glutamyltransferase Transferase Periplasm 575 18 6 14
303c 23c 11c 19c
A1VXC2 (Cj0037c) Putative cytochrome C Electron carrier activity Periplasm 46 1 1 5
A1VXM7 (Cj0143c) Putative periplasmic solute binding protein for ABC transporter system Metal ion binding Periplasm 36 1 1 4
A1VY79 (Cj0358) Putative cytochrome C551 peroxidase Peroxidase Periplasm 627 33 3 13
283c 22c 13c 38c
A1W0L1 (Cj1228c) Serine protease Proteolysis Periplasm 239 11 8 31
A8FKK2 (Cj0415) Putative GMC oxidoreductase subunit Unknown Unknown 1830 71 16 38
286c 15c 13c 28c

*Gene designation refers to the best match identified in Campylobacter jejuni NCTC 11168 (where present).

a The programme signal was used for protein localisation prediction; non-cytoplasmic localisation refers to proteins whose localisations are unknown, but a cytoplasmic localisation is ruled out as a N-terminal signalling sequence is present.

b The uniprot annotation was used for protein localisation prediction.

c The identification data obtained from the gel based analysis.

Prediction of protein localisation was determined using the program PSORTb, and table listings are sorted by localisation.

Gatsos et al.

Gatsos et al. BMC Microbiology 2012 12:303   doi:10.1186/1471-2180-12-303

Open Data