Figure 2.

Schematic diagram and SDS-PAGE analysis of expressed PlyBt33 and its functional domains.(a) Schematic diagram of expressed PlyBt33 (full length), PlyBt33-N (N-terminal), and PlyBt33-IC (IC-terminal) proteins. The numbers above the rectangle correspond to amino acid residues. (b) SDS-PAGE analysis of expressed and purified PlyBt33, PlyBt33-N, and PlyBt33-IC proteins. Marker, molecular mass marker; lane 1, Ni-NTA column-purified PlyBt33 from E. coli supernatant following ultrasonication; lane 2, Ni-NTA column-purified PlyBt33-N from E. coli supernatant following ultrasonication; lane 3, Ni-NTA column-purified PlyBt33-IC from E. coli supernatant following ultrasonication. PlyBt33, PlyBt33-N, and PlyBt33-IC bands appeared at 33 kDa, 24 kDa, and 11 kDa, respectively.

Yuan et al. BMC Microbiology 2012 12:297   doi:10.1186/1471-2180-12-297
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