Transcriptional regulation of icaA and icaR by AI-2. Real-time RT-PCR of icaA and icaR transcription was measured. The bacteria used for RNA extraction were those that were incubated at 4 h for biofilm formation under aerobic conditions. Error bars indicate the variation between triplicate samples within the real-time RT-PCR. The relative cDNA abundance of the WT sample was assigned a value of 1. (A) Relative transcript levels of icaA of WT (RN6390B), ΔluxS and ΔluxS complemented with 3.9 nM DPD under aerobic conditions. (B) Relative transcript levels of icaR of WT (RN6390B), ΔluxS and ΔluxS complemented with 3.9 nM DPD under aerobic conditions.
Yu et al. BMC Microbiology 2012 12:288 doi:10.1186/1471-2180-12-288