Biofilm formation in flow cell and chemical complementation by DPD. Biofilms of WT (RN6390BG) and ΔluxS (ΔluxSG) were grown in a flow cell in 2% TSB with chloramphenicol (15 μg/ml). Biofilm integrity and GFP fluorescence were monitored at the 3rd day and the 5th day by CLSM. For chemical complementation, 3.9 nM DPD was added to the TSB medium at the beginning of the experiment. CLSM images are representative of two separate experiments and each grid square represents 20 μm (A) WT. (B) ΔluxS. (C) WT supplemented with DPD. (D) ΔluxS supplemented with DPD.
Yu et al. BMC Microbiology 2012 12:288 doi:10.1186/1471-2180-12-288