Open Access Research article

Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions

Aurélie Crabbé145, Baptiste Leroy2, Ruddy Wattiez2, Abram Aertsen3, Natalie Leys1, Pierre Cornelis4 and Rob Van Houdt1*

Author Affiliations

1 Unit of Microbiology, Expert Group Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium

2 Department of Proteomics and Microbiology, Interdisciplinary Center of Mass Spectrometry (CISMa), University of Mons (UMONS), Mons, Belgium

3 Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Centre, Centre for Food and Microbial Technology, Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium

4 Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Institute for Biotechnology (VIB), Vrije Universiteit Brussel, Brussels, Belgium

5 Present address: The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, 1001 S. McAllister Avenue, Tempe, AZ, 85287, USA

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BMC Microbiology 2012, 12:282  doi:10.1186/1471-2180-12-282

Published: 27 November 2012

Additional files

Additional file 1:

Figure S1. Morphologic analysis of a P. putida KT2440 isogenic recA mutant grown at 50 rpm and 150 rpm. Flow cytometry dot plot (forward scatter versus side scatter) of P. putida KT2440 recA mutant grown at 50 rpm (A) and 150 rpm (B). Microscopic imaging of Hoechst-stained P. putida KT2440 recA mutant grown at 50 rpm (C) and 150 rpm (D) (magnification = 1000x). Flow cytometry histogram of P. putida KT2440 recA mutant grown at 50 rpm (grey line) and 150 rpm (black line) (E), representing the average bacterial length.

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Additional file 2:

Figure S2. 3 Heat shock resistance of a P. putida KT2440 isogenic recA mutant grown at 50 and 150 rpm, as compared to wild type. Bacteria were exposed to 55°C during 30 min.

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