Open Access Methodology article

Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

Marcelo Mendonça123, Neida L Conrad1, Fabricio R Conceição1, Ângela N Moreira1, Wladimir P da Silva2, José AG Aleixo1* and Arun K Bhunia3*

Author Affiliations

1 Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, 96010-900 Pelotas, RS, Brazil

2 Laboratório de Microbiologia de Alimentos, Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, 96010-900, Pelotas, RS, Brazil

3 Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA

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BMC Microbiology 2012, 12:275  doi:10.1186/1471-2180-12-275

Published: 23 November 2012

Additional files

Additional file 1:

Figure S1. Indirect immunofluorescence assay of L. monocytogenes (top row) and L. innocua (bottom row) immunoprobed with anti-InlA MAb-2D12 and FITC-conjugated anti-mouse antibodies. Cells were counter-stained with Hoechst for nuclear staining to assess the total bacterial cells. Magnification, 1000×.

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Additional file 2:

Figure S2. Capture efficiency of MyOne-2D12 (InlA), MyOne-3F8 (p30), and Dynabeads anti-Listeria (Dynal) from soft cheese inoculated with L. monocytogenes and L. innocua and enriched in FB. Captured cells were plated on (a) MOX plates for enumeration and (b) BHI for confirmation of L. monocytogenes (Lm) and L. innocua (Linn) counts by a light-scattering sensor, BARDOT.

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Additional file 3:

Table S1. Description of bacterial strains used.

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