Table 1

Bacterial strains and plasmids
Strains Relevant Genotype Origin or reference
C-1a E. coli C, prototrophic [40]
C-5691 Δpnp-751 [41]
C-5928 ΔbcsA::cat by P1 HTF AM72 transduction into C-1a
C-5929 Δpnp-751 ΔbcsA::cat by P1 HTF AM72 transduction into C-5691
C-5930 ΔcsgA::cat by P1 HTF AM70 transduction into C-1a
C-5931 Δpnp-751 ΔcsgA::cat by P1 HTF AM70 transduction into C-5691
C-5932 ΔpgaA::cat by P1 HTF AM56 transduction into C-1a
C-5933 Δpnp-751 ΔpgaA::cat by P1 HTF AM56 transduction into C-5691
C-5934 ΔwcaD::tet by P1 HTF AM105 transduction into C-1a
C-5935 Δpnp-751 ΔwcaD::tet by P1 HTF AM105 transduction into C-5691
C-5936 ΔpgaC::kan by P1 HTF JW1007 transduction into C-1a
C-5937 Δpnp-751 ΔpgaC::kan by P1 HTF JW1007 transduction into C-5691
C-5938 ΔcsrA::kan From C-1a by λ Red-mediated recombination; primers: FG2624 and FG2625
C-5940 ΔcsrB::kan From C-1a by λ Red-mediated recombination; primers: FG2524 and FG2525
C-5942 Δpnp-751 ΔcsrB::kan From C-5691 by λ Red-mediated recombination; primers: FG2524 and FG2525.
C-5944 ΔcsrC::cat From C-1a by λ Red-mediated recombination; primers: FG2585 and FG2586.
C-5946 Δpnp-751 ΔcsrC::cat From C-5691 by λ Red-mediated recombination; primers: FG2585 and FG2586.
C-5948 ΔcsrB::kan ΔcsrC::cat by P1 HTF C-5940 transduction into C-5944
C-5950 Δpnp-751 ΔcsrB::kan ΔcsrC::cat by P1 HTF C-5940 transduction into C-5946
C-5952 ΔcsrD::cat From C-1a by λ Red-mediated recombination; primers: PL674 and PL675.
C-5954 Δpnp-751 ΔcsrD::cat From C-5691 by λ Red-mediated recombination; primers: PL674 and PL675.
C-5960 ΔmcaS::kan From C-1a by λ Red-mediated recombination; primers: FG2755 and FG2756.
C-5962 Δpnp-751 ΔmcaS::kan From C-5691 by λ Red-mediated recombination; primers: FG2755 and FG2756.
JW1007 BW25113 ΔpgaC::kan [68]
AM56 MG1655 ΔpgaA::cat [69]
AM70 MG1655 ΔcsgA::cat [69]
AM72 MG1655 ΔbcsA::cat [69]
AM105 MG1655 ΔwcaD::tet From MG1655 by λ Red-mediated recombination with a DNA fragment obtained by PCR of tet10 cassette of EB 1.3 with primers PL372 and PL373.
EB 1.3 MG1655 rpoS::Tn10-tet [33]
Plasmids and phage Relevant characteristics Reference
pBAD24 AmpR, ColE1 [70]
pBAD24-Δ1 pBAD24 derivative with a modified polylinker; carries an unique NcoI site overlapping the araBp transcription start this work
pBADpnp pBAD24 derivative; harbours an EcoRI-HindIII fragment of pEJ01 that carries the pnp gene this work
pBADrnb pBAD24 derivative; harbours an HindIII-XbaI fragment of pFCT6.9 that carries the rnb gene this work
pBADrnr pBAD24-Δ1 derivative; harbours the rnr gene (obtained by PCR on MG1655 DNA with FG2474-FG2475 oligonucleotides) between NcoI-HindIII sites this work
pΔLpga pJAMA8 derivative, harbours the -116 to +32 region relative to the pgaABCD transcription start site cloned into the SphI/XbaI sites this work
pEJ01 carries a His-tagged pnp allele [71]
pFCT6.9 carries a His-tagged rnb allele [72]; received from Cecilia Arraiano
pGZ119HE oriVColD; CamR [73]
pJAMA8 AmpR, ColE1; luxAB based promoter-probe vector. [37]
pLpga1 pJAMA8 derivative, harbours the -116 to +234 region relative to the pgaABCD transcription start site cloned into the SphI/XbaI sites. this work
pLpga2 pJAMA8 derivative, harbours a translational fusion of pgaA promoter, regulatory region and first 5 codons of pgaA (-116 to +249 relative to transcription start site) with luxA ORF (Open Reading Frame). this work
pTLUX pJAMA8 derivative, harbours ptac promoter of pGZ119HE cloned into the SphI/XbaI sites. this work
P1 HTF High transduction frequency phage P1 derivative [74]; received from Richard Calendar

Carzaniga et al.

Carzaniga et al. BMC Microbiology 2012 12:270   doi:10.1186/1471-2180-12-270

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