Table 1

Strains and plasmids used in this study
Strain Relevant characteristic Source or reference
Escherichia coli JM109 Cloning strain
E. coli S17-1 Conjugation strain [45]
Ralstonia eutropha H16 Wild type strain, PHB accumulation DSMZ 428
Ralstonia eutropha HF39 Streptomycin resistant derivate of H16 [22,39]
R. eutropha H16 ∆phaP5 Chromosomal deletion of phaP5 [22]
R. eutropha H16 ∆phaM Chromosomal deletion of phaM [32]
Plasmid Relevant feature(s) Source or reference
pBBR1MCS-2 broad host range vector [46]
pBBR1MCS2- PphaC-eyfp-c1 Constitutive eYfp over-expression [22]
pBBR1MCS-2- PphaC-eyfp-phaP5 Fusion of PhaP5 to C-terminus of eYfp [22]
pBBR1MCS-2- PphaC–eyfp-phaM Fusion of eYfp to N-terminus to PhaM [32]
pBBR1MCS-2- PphaC–phaP5 Constitutive over-expression of PhaP5 this study
pBBR1MCS-2- PphaC–phaM Constitutive over-expression of PhaM this study

Wahl et al.

Wahl et al. BMC Microbiology 2012 12:262   doi:10.1186/1471-2180-12-262

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