Table 4

Expression of LEE encoded, flagellar and stx2 genes in presence of 100 μg/ml limonoids
Gene name Ichangin Isolimonic acid Isoobacunoic acid IOAG DNAG
ler -3.2 (±2.1) -5.0 (±0.8) -1.4 (±0.3) -1.8 (±0.4) -0.7 (±1.5)
escU -5.3 (±0.8) -6.6 (±1.0) -1.6 (±0.1) -3.2 (±0.3) -2.0 (±0.6)
escR -2.5 (±0.7) -6.3 (±1.3) -1.7 (±0.3) -2.5 (±1.2) -2.3 (±0.5)
escJ -6.2 (±1.0) -12.4 (±2.1) -2.4 (±1.3) -3.7 (±2.0) -1.2 (±2.4)
sepZ -2.7 (±0.1) -6.9 (±1.1) -0.7 (±1.5) -1.7 (±0.6) -1.6 (±0.8)
cesD -3.5 (±0.7) -10.0 (±1.5) -3.0 (±1.2) -2.6 (±1.7) -1.6 (±0.8)
flhC -2.8 (±0.9) -4.5 (±1.3) -1.5 (±0.3) -2.1 (±0.4) -1.3 (±0.3)
flhD -2.8 (±0.5) -6.9 (±0.4) -1.8 (±0.5) -2.3 (±0.4) -1.7 (±0.5)
stx2 -2.5 (±0.8) -4.9 (±1.0) -1.6 (±0.4) -2.2 (±0.8) -1.2 (±0.1)
rpoA -0.3 (±1.8) -0.5 (±1.6) 1.8 (±0.8) 1.3 (±0.4) 1.7 (±0.5)

The EHEC ATCC 43895 was grown to OD600≈1.0, RNA was extracted using RNeasy kit and converted to cDNA as described in text. Target genes were amplified from three biological samples. Fold change was calculated using 2(−ΔΔCt) method and presented as mean ± SD of three replicates.

Vikram et al.

Vikram et al. BMC Microbiology 2012 12:261   doi:10.1186/1471-2180-12-261

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