Table 1

Escherichia coli K-12 strains

Strain

Relevant genotype

Source


General P1 donors


VS111

F- ΔtopA75 zci2234::cat Δfnr-267? rph-1

CGSC [11]


MG1655 and derivatives


N4560

ΔrecG::cat

[26]


N4704

rnhA::cat

[15]


N6052

ΔrecG::apra

[13]


AM2283

ΔlacIZYA ΔproB::rnhA+-frt > kan > frt

This study


AM2284

ΔlacIZYA ΔproB::rnhA+- frt > kan > frt pCP20

AM2283 × pCP20 to Apr


AM2285

ΔlacIZYA ΔproB::rnhA+- frt

AM2284


AM2290

ΔlacIZYA ΔproB::rnhA+- frt > kan > frt

TB28 × P1.AM2283 to Kmr


AM2304

ΔlacIZYA ΔproB::rnhA+- frt > kan > frt ΔrecG::apra

AM2290 × P1.N6052 to Aprar


AS1047

ΔlacIZYA pAST111

TB28 × pAST111 to Apr


AS1050

ΔlacIZYA ΔtopA::apra pAST111

AS1047 × P1.RCe296 to Aprar


AS1053

ΔlacIZYA topA::apra ΔrecG::cat pAST111

AS1050 × P1.N4560 to Cmr


AS1054

ΔlacIZYA topA::apra rnhA::cat pAST111

AS1050 × P1.N4704 to Cmr


AS1066

ΔlacIZYA topA::apra pAST111 pECR17

AS1050 × pECR17 to Apr Kmr


AS1067

ΔlacIZYA topA::apra ΔrecG::cat pAST111 pECR17

AS1053 × pECR17 to Apr Kmr


AS1068

ΔlacIZYA topA::apra rnhA::cat pAST111 pECR17

AS1054 × pECR17 to Apr Kmr


AS1070

ΔlacIZYA ΔtopA75 zci-2234::cat pAST111

AS1047 × P1.VS111 to Cmr


AS1130

ΔlacIZYA ΔproB::rnhA+-frt pAST111

AM2285 × pAST111 to Apr


AS1131

ΔlacIZYA ΔproB::rnhA+-frt topA::apra pAST111

AS1130 × P1.RCe296 to Aprar


AS1133

ΔlacIZYA topA::apra pAST111 pAST120

AS1050 × pAST120 to Kmr (Apr)


AS1134

ΔlacIZYA ΔproB::rnhA+- frt > kan > frt ΔrecG::apra pJJ100

AM2304 × pJJ100 to Apr


AS1137

ΔlacIZYA ΔproB::rnhA+- frt > kan > frt ΔrecG::apra rnhA::cat pJJ100

AS1134 × P1.N4704 to Cmr


AS1139

ΔlacIZYA ΔproB::rnhA+- frt topA::apra pAST111 pECR17

AS1131 × pERC17 to Kmr (Apr)


RCe296

topA::apra

This study


TB28

ΔlacIZYA

[12]


Stockum et al. BMC Microbiology 2012 12:26   doi:10.1186/1471-2180-12-26

Open Data