Additional file 2.

Figure S2. The viability of cells increased levels of RNase HI is reduced. Wild type cells carrying a ParaBAD rnhA expression plasmid (pECR15) show a growth defect that depends on the concentration of arabinose present in the growth medium. Even growth on glucose, which suppresses expression from the ParaBAD promoter, leads to a mild growth defect, presumably due to a combination of the high plasmid copy number and the leakiness of the ParaBAD promoter. Cells carrying a control plasmid (ParaBAD eCFP, pAST110) show no growth restriction.

Format: PDF Size: 447KB Download file

This file can be viewed with: Adobe Acrobat Reader

Stockum et al. BMC Microbiology 2012 12:26   doi:10.1186/1471-2180-12-26