Analysis of plasmid content of Mycoplasma yeatsii type strain GIH (TS).A. Agarose gel electrophoresis of total DNA. Lanes were loaded after twofold dilution series of the DNA preparation obtained as described in Methods. Bands corresponding to the chromosome and the 2 plasmids are identified. Lane M, DNA ladder. B. Estimated plasmid copy number of pMyBK1 and pMG2B-1 as estimated by gel assay (Panel A) and relative real-time PCR as described in Methods.
Breton et al. BMC Microbiology 2012 12:257 doi:10.1186/1471-2180-12-257