Structural organization and replication ability of pMyBK1 and derivatives.A. Plasmid constructs are described in Methods. Putative promoter and terminator of CDSA and CDSB are indicated for pMyBK1 only. Direct repeats (□) , inverted repeats (▸◂) and the GC-rich region (|||||) are indicated only for the pCM-C derivative. B, BglII; E, EcoRI; spi, Spiroplasma citri spiralin gene; tetM, tetracycline resistance gene from transposon Tn916, pBS, plasmid pBluescript. The signs on the right indicate the ability (+) and inability (−) to replicate in Mycoplasma yeatsii type strain GIH TS. * indicates a frameshift mutation in the cdsB sequence of pCM-K1ΔB. B. The replication ability of 4 pMyBK1 derivatives was evaluated in mollicute species belonging to the Spiroplasma phylogenetic group and shown to be initially plasmid-free: M. yeatsii #13156, M. putrefaciens KS1 TS, M. leachii PG50 TS, Mcc California kid TS, Mmc GM12, Mmm T1/44 and S. citri GII3. The signs on the right indicate the ability (+) and inability (−) to replicate in a given species. ND: not determined. A: plasmid integration in the Mmc chromosome. B: spiralin expression in Mcc was detected by immunoblot.
Breton et al. BMC Microbiology 2012 12:257 doi:10.1186/1471-2180-12-257