Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces species
Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China
BMC Microbiology 2012, 12:253 doi:10.1186/1471-2180-12-253Published: 7 November 2012
Additional file 1:
Figure S1. Identification of fourteen indigenous plasmids. Fourteen plasmids from endophytic Streptomyces strains were digested with NcoI and electrophoresed in 1% agarose gel at 6.7 V/cm for 4 h. Sizes of five bands are indicated.
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Additional file 2:
Figure S2. Features of the 1136-bp sequence of the Y27 chromosomal oriC between the dnaA and dnaN genes. Taking the conserved DnaA binding-boxes of 9 bp (TTGTCCACA) in the S. lividans oriC as a reference , 25 DnaA binding-boxes of 9 bp (forward indicated by arrowheads and reverse by dashed arrowheads) for the Y27 oriC are predicted by the Vector NTI® 9.0 software (Invitrogen). Two AT-rich sequences are boxed.
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Additional file 3:
Figure S3. Identification of fourteen endophytic Streptomyces strains. The plug-embedded mycelium of fourteen endophytic Streptomyces strains was digested with SspI and electrophoresed in a 1.0% pulsed-field gel at 8.6 V/cm, 10 s to 60 s switch time and 14oC for 22 h.
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Additional file 4:
Figure S4. Schematic map of pWTY27. Predicted ORFs and their transcription directions are indicated by arrowheads. The replication (repA and repB), transfer (traA) and other genes (int: integrase; phc: phage capsid; kor: kill-override; spd: spread) and site (iteron) are shown. (JPEG 32 kb)
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