Open Access Research article

Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces species

Tao Wang, Zhenhua Chen, Qiuxiang Cheng, Min Zhou, Xinli Tian, Pengfei Xie, Li Zhong, Meijuan Shen and Zhongjun Qin*

Author Affiliations

Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China

For all author emails, please log on.

BMC Microbiology 2012, 12:253  doi:10.1186/1471-2180-12-253

Published: 7 November 2012

Additional files

Additional file 1:

Figure S1. Identification of fourteen indigenous plasmids. Fourteen plasmids from endophytic Streptomyces strains were digested with NcoI and electrophoresed in 1% agarose gel at 6.7 V/cm for 4 h. Sizes of five bands are indicated.

Format: JPEG Size: 106KB Download file

Open Data

Additional file 2:

Figure S2. Features of the 1136-bp sequence of the Y27 chromosomal oriC between the dnaA and dnaN genes. Taking the conserved DnaA binding-boxes of 9 bp (TTGTCCACA) in the S. lividans oriC as a reference [24], 25 DnaA binding-boxes of 9 bp (forward indicated by arrowheads and reverse by dashed arrowheads) for the Y27 oriC are predicted by the Vector NTI® 9.0 software (Invitrogen). Two AT-rich sequences are boxed.

Format: JPEG Size: 248KB Download file

Open Data

Additional file 3:

Figure S3. Identification of fourteen endophytic Streptomyces strains. The plug-embedded mycelium of fourteen endophytic Streptomyces strains was digested with SspI and electrophoresed in a 1.0% pulsed-field gel at 8.6 V/cm, 10 s to 60 s switch time and 14oC for 22 h.

Format: JPEG Size: 94KB Download file

Open Data

Additional file 4:

Figure S4. Schematic map of pWTY27. Predicted ORFs and their transcription directions are indicated by arrowheads. The replication (repA and repB), transfer (traA) and other genes (int: integrase; phc: phage capsid; kor: kill-override; spd: spread) and site (iteron) are shown. (JPEG 32 kb)

Format: JPEG Size: 32KB Download file

Open Data

Additional file 5:

Table S1. Predicted ORFs of plasmid pWTY27. Detailed information and possible functions of the fifteen ORFs of pWTY27.

Format: DOC Size: 39KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data