Open Access Highly Accessed Research article

Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species

Joshua K Stone1, Mark Mayo2, Stephanie A Grasso1, Jennifer L Ginther1, Stephanie D Warrington1, Christopher J Allender1, Adina Doyle1, Shalamar Georgia1, Mirjam Kaestli2, Stacey M Broomall3, Mark A Karavis3, Joseph M Insalaco3, Kyle S Hubbard3, Lauren A McNew3, Henry S Gibbons3, Bart J Currie2, Paul Keim1 and Apichai Tuanyok1*

Author Affiliations

1 Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ, USA

2 Menzies School of Health Research, Darwin, NT, Australia

3 BioSciences Division, Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD, USA

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BMC Microbiology 2012, 12:250  doi:10.1186/1471-2180-12-250

Published: 5 November 2012

Additional files

Additional file 1:

Table S1. List of Burkholderia strains used in this study, and their identified genotypes and phenotypes.

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Additional file 2:

Figure S1. SDS-PAGE and immunoblotting analyses of 3 reference LPS banding patterns A, B, and B2 in B. pseudomallei strains K96243 (lane 1), 576 (lane 2), and MSHR840 (lane 3), respectively. Panel A is the silver stained SDS-PAGE. Panels B and C are the immunoblots of LPS samples in panel A which were hybridized against sera from serotype A and B patients, respectively. Lane 4 is the LPS from B. pseudomallei strain MSHR1655 which is rough type and not seroreactive. Lane L is a standard protein ladder.

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Additional file 3:

Figure S2. Comparison of type A O-antigen biosynthesis clusters. Type A O-antigen is found in four species, from top to bottom, B. oklahomensis, B. pseudomallei, B. mallei, and B. thailandensis. Red indicates nucleotide homology of 78-100%. The glycosyltransferase gene wbiE (BoklE_010100014785) is truncated in B. oklahomensis E0147 but maintains functional. Conversely, insertion of a thymine into the methyltransferase wbiD relative to B. pseudomallei K96243 removes the functionality of this enzyme in E0147, removing it from the comparison.

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