Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Relevant genotype and/or description Source or reference
X. campestrispv. campestris strains
B100 Wild-type, Smr [46]
B100-6.01 Control strain, carrying ΩKm(cat) in intergenic region flanked by tonB1 and exbB1, Smr, Kmr [64]
B100-5.05 tonB1-deficient mutant, Smr, Kmr [64]
B100-7.03 exbB1-deficient mutant, Smr, Kmr [64]
B100-9.01 exbD1-deficient mutant, Smr, Kmr [64]
B100-11.03 exbD2-deficient mutant, Smr, Kmr [64]
B100-Bac2 Bacitracin-resistant spontaneous mutant of B100, unable to produce polysaccharides, Smr D. Steinmann, CeBiTec culture collection
E. colistrain
XL1Blue recA1, thi, supE44, lac, [F’proAB lacIq, lacZΔM15, Tn10(Tcr)] [89]
Plasmids
pUC6S lacZα, Apr [90]
pBCKS+ pUC19, lacZ, Cmr Stratagene
pBCSK+ pUC19, lacZ, Cmr Stratagene
pMS246 pSVB30, aacC1, Gmr [91]
pHGW31 pHIP, aacC1ΔBglII, Gmr [64]
pHGW241 pHGW31, tonB1, Gmr [64]
pHGW242 pHGW31, exbB1, Gmr [64]
pHGW243 pHGW31, exbD1, Gmr [64]
pHGW244 pHGW31, exbD2, Gmr [66]
pIJ3051 pLAFRI-based cosmid carrying 27.9 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Tcr [39]
pHGW260 pHGW31, 11.1 kb chromosomal BamHI fragment of X. campestris pv. campestris 8004 with pglI, Gmr This study
pHGW261 pBCKS+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr This study
pHGW262 pBCSK+, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260, Cmr This study
pHGW267 pUC6S, 3.8 kb BamHI-ClaI subfragment with pglI from pHGW260 expressed from aacC1 Pout promoter, Apr, Gmr This study

Vorhölter et al.

Vorhölter et al. BMC Microbiology 2012 12:239   doi:10.1186/1471-2180-12-239

Open Data