Oxidative burst reactions in heterologous N. tabacum cell suspension cultures after elicitation with isolated OGAs. To functionally characterize OGAs differing in their DPs they were checked for their capacity to evoke oxidative burst reactions in cell-suspension cultures of the non-host plant N. tabacum. Samples of the OGAs were added to the cell suspension cultures to a final concentration of 5 μg/ml. The amount of H2O2 produced upon the addition of the OGAs was monitored as described before. The addition of water used as negative control (♦) had no effect, and OGAs with a DP of 2 (■), a DP of 3 (●), a DP of 4 (▲), and a DP of 6 (◊) had only minimal effects on the N. tabacum suspension cells. The response to OGAs with DPs of 5 (✶), 7 (□), and 8 (∆) was slightly stronger but still small. But for OGAs whereof the DP exceeded 8 (○), a clear oxidative burst reaction was observed. This indicated the largest OGA fraction as elicitor of the non-host plant defense against X. campestris pv. campestris.
Vorhölter et al. BMC Microbiology 2012 12:239 doi:10.1186/1471-2180-12-239