Table 1

Strains and plasmids used in this study
Strain Plasmid Promoter Gene # if isolates Description
WT 11 Control
WT pSET152 pSET152 22 Control
WT allN pDG1 PermE* allN 28 Over-expression
WT allN+mgl pDG2 PermE* allN+mgl 25 Over-expression
WT fkbR pDG3 PermE* fkbR 24 Over-expression
WT fkbN pDG4 PermE* fkbN 29 Over-expression
ΔallN pDG5 ΔallN 7 Gene inactivation
ΔallNΔfkbN pDG5, 8 ΔallN ΔfkbN 2 Gene inactivation
ΔfkbN pDG8 ΔfkbN 10 Gene inactivation
ΔfkbR pDG6 ΔfkbR 6 Gene inactivation
ΔfkbRΔfkbN pDG6, 8 ΔfkbR ΔfkbN 2 Gene inactivation
ΔfkbR pDG3 PermE* fkbR 18 Complementation
ΔfkbN pDG4 PermE* fkbN 33 Complementation
ΔfkbRΔfkbN pDG4 PermE* fkbN 9 Complementation
WT pSET152 rppA 12 Negative control
ΔfkbR pMB1 PermE* rppA 9 Positive control
pMB2 PallA rppA 15 Promoter activity
pMB3 PfkbR rppA 15 Promoter activity
ΔfkbN pMB4 PfkbN rppA 12 Promoter activity
pMB5 PfkbB rppA 15 Promoter activity
pMB6 PfkbG rppA 15 Promoter activity

Goranovič et al.

Goranovič et al. BMC Microbiology 2012 12:238   doi:10.1186/1471-2180-12-238

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