Open Access Highly Accessed Research article

FK506 biosynthesis is regulated by two positive regulatory elements in Streptomyces tsukubaensis

Dušan Goranovič1, Marko Blažič2, Vasilka Magdevska1, Jaka Horvat1, Enej Kuščer15, Tomaž Polak2, Javier Santos-Aberturas3, Miriam Martínez-Castro3, Carlos Barreiro3, Peter Mrak4, Gregor Kopitar4, Gregor Kosec15, Štefan Fujs1, Juan F Martín3 and Hrvoje Petković12*

Author Affiliations

1 Acies Bio d.o.o., Tehnološki Park 21, SI-1000, Ljubljana, Slovenia

2 Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia

3 Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Avenida. Real, No. 1, 24006, León, Spain

4 Lek Pharmaceuticals d.d., a Sandoz company, Verovškova 57, SI-1526, Ljubljana, Slovenia

5 Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, (CIPKeBiP), Jamova 39, Ljubljana, Slovenia

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BMC Microbiology 2012, 12:238  doi:10.1186/1471-2180-12-238

Published: 19 October 2012

Additional files

Additional file 1:

Table containing primers for PCR amplifications of the target putative regulatory genes (The file presents primers and their corresponding sequences, that have been used for PCR amplification of whole genes or homologous regions and promoter regions).

Format: PDF Size: 41KB Download file

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Open Data

Additional file 2:

Schematic representation of FkbR and FkbN protein domains and deleted regions (This file illustrates FkbR and FkbN proteins and their organization before and after inactivation).

Format: PDF Size: 13KB Download file

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Open Data

Additional file 3:

Primers used for RT-PCR analysis (This file presents a list of primers and their corresponding sequences, that have been used for RT-PCR experiments).

Format: PDF Size: 42KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data