Table 2

qPCR data of Lactobacillus, Bifidobacterium, Atopobium and Prevotella
ng of target DNA/μg vaginal genomic DNA (mean ± SD)
Woman N. Time point Lactobacillus Bifidobacterium Atopobium Prevotella
Probiotic (P)
1 W33 2.4 × 101 ± 1.1 1.9 × 10-2 ± 7.4 × 10-3 3.6 ± 1.5 2.1 × 10-2 ± 1.0 × 10-2
W37 3.0 × 101 ± 3.1 3.1 × 10-2 ± 2.7 × 10-4 1.3 × 101 ± 6.8 9.1 × 10-2 ± 1.6 × 10-2
2 W33 9.6 ± 8.7 × 10-1 3.1 × 10-2 ± 8.8 × 10-3 5.4 × 101 ± 7.4 1.4 × 10-1 ± 4.8 × 10-2
W37 5.9 × 10-1 ± 4.9 × 10-2 2.4 × 10-2 ± 1.2 × 10-2 2.4 × 101 ± 1.9 × 101 1.1 × 10-1 ± 1.1 × 10-2
3 W33 2.4 × 101 ± 2.9 2.4 × 10-2 ± 4.2 × 10-3 1.1 × 101 ± 6.0 1.1 × 10-1 ± 7.7 × 10-3
W37 2.2 × 101 ± 2.4 3.0 × 10-2 ± 2.4 × 10-3 4.0 ± 2.3 5.2 × 10-2 ± 8.2 × 10-3
4 W33 2.2 × 101 ± 2.0 6.8 × 10-2 ± 8.3 × 10-3 4.7 ± 1.9 7.3 × 10-2 ± 2.9 × 10-2
W37 1.5 × 101 ± 1.4 2.1 × 10-2 ± 7.2 × 10-3 5.2 ± 2.0 4.6 × 10-2 ± 9.5 × 10-3
5 W33 2.5 × 101 ± 4.5 2.1 × 10-2 ± 3.4 × 10-3 1.2 × 101 ± 3.0 9.3 × 10-2 ± 8.3 × 10-3
W37 2.2 x 101 ± 4.5 1.4 x 10-2 ± 3.2 x 10-3 1.5 x 101 ± 1.9 3.0 x 10-2 ± 1.1 x 10-2
6 W33 1.1 × 10-1 ± 3.4 × 10-3 7.1 × 10-2 ± 7.1 × 10-3 1.0 × 101 ± 4.1 1.2 × 10-1 ± 1.3 × 10-2
W37 2.2 ± 6.0 × 10-1 2.1 ± 1.7 × 10-1 2.4 × 101 ± 1.0 × 101 1.5 × 10-1 ± 1.2 × 10-2
7 W33 4.1 × 101 ± 8.5 3.7 × 10-2 ± 5.4 × 10-3 2.9 × 101 ± 9.2 1.2 × 10-1 ± 2.1 × 10-2
W37 2.0 × 101 ± 2.6 1.7 × 10-2 ± 4.4 × 10-3 2.6 × 101 ± 7.7 1.1 × 10-1 ± 1.1 × 10-3
8 W33 1.0 × 101 ± 1.7 × 10-1 1.3 × 10-2 ± 1.9 × 10-3 5.5 ± 1.2 4.2 × 10-2 ± 1.9 × 10-2
W37 2.1 × 101 ± 2.0 1.5 × 10-2 ± 2.6 × 10-3 1.6 × 101 ± 6.6 5.1 × 10-2 ± 3.3 × 10-3
9 W33 0.0 ± 0.0 7.1 × 10-3 ± 2.8 × 10-5 1.8 × 101 ± 7.1 6.7 × 10-2 ± 1.5 × 10-2
W37 0.0 ± 0.0 1.1 × 101 ± 1.0 1.5 × 101 ± 6.8 2.3 × 10-1 ± 8.0 × 10-2
10 W33 6.7 ± 6.1 × 10-1 2.0 × 10-2 ± 4.8 × 10-3 1.4 × 101 ± 4.3 8.6 × 10-2 ± 2.0 × 10-2
W37 1.1 × 101 ± 1.4 2.3 × 10-2 ± 1.5 × 10-2 1.7 × 101 ± 9.7 8.0 × 10-2 ± 2.9 × 10-2
11 W33 2.7 × 101 ± 1.7 2.9 x 10-3 ± 1.7 × 10-3 2.3 ± 1.8 3.2 × 10-2 ± 3.3 × 10-3
W37 3.0 × 101 ± 5.6 1.3 x 10-2 ± 8.5 × 10-3 1.3 ± 7.5 × 10-1 3.6 × 10-2 ± 1.3 × 10-2
12 W33 2.2 ± 5.6 × 10-1 1.5 × 101 ± 2.3 1.4 × 101 ± 2.9 2.2 × 10-1 ± 2.1 × 10-2
W37 2.0 ± 3.1 × 10-1 8.7 ± 5.6 × 10-1 1.2 × 101 ± 2.3 1.0 × 10-1 ± 1.8 × 10-2
13 W33 3.7 × 101 ± 5.4 3.0 × 10-2 ± 4.5 × 10-3 7.0 ± 2.6 × 10-1 2.7 × 10-2 ± 5.0 × 10-4
W37 6.6 × 101 ± 5.9 1.1 × 10-2 ± 2.2 × 10-3 6.8 ± 6.6 × 10-1 5.7 × 10-2 ± 2.0 × 10-3
14 W33 2.2 × 101 ± 8.5 1.7 × 10-2 ± 4.9 × 10-3 9.0 ± 4.4 × 10-1 6.7 × 10-2 ± 6.6 × 10-3
W37 1.6 × 101 ± 4.9 2.8 × 10-2 ± 4.7 × 10-3 1.1 × 101 ± 1.1 1.1 × 10-1 ± 1.8 × 10-3
15 W33 2.2 × 101 ± 7.1 1.4 × 10-2 ± 7.1 × 10-3 1.8 × 101 ± 5.6 1.1 × 10-1 ± 1.4 × 10-2
W37 2.8 × 101 ± 3.4 4.7 × 10-3 ± 2.3 × 10-3 1.1 × 101 ± 2.4 × 10-1 7.4 × 10-2 ± 2.4 × 10-3
Control (C)
16 W33 5.4 × 101 ± 4.0 2.1 × 10-2 ± 5.6 × 10-3 1.1 × 101 ± 4.6 6.8 × 10-2 ± 1.1 × 10-2
W37 2.0 × 101 ± 1.7 2.0 × 10-2 ± 7.4 × 10-3 1.4 × 101 ± 5.0 5.6 × 10-2 ± 5.4 × 10-3
17 W33 5.5 ± 5.3 × 10-1 6.0 ± 1.6 × 10-1 1.2 × 101 ± 4.3 5.9 × 10-2 ± 2.3 × 10-2
W37 1.5 × 101 ± 2.9 9.3 ± 5.3 × 10-1 1.9 × 101 ± 8.7 5.4 × 10-2 ± 1.0 × 10-2
18 W33 2.6 ± 1.6 × 10-1 1.8 ± 3.5 × 10-2 1.3 × 101 ± 5.5 8.8 × 10-2 ± 1.7 × 10-2
W37 1.2 × 101 ± 2.0 2.9 ± 7.5 × 10-2 3.3 × 101 ± 4.4 4.5 × 10-2 ± 2.8 × 10-3
19 W33 7.6 × 101 ± 3.3 × 10-1 1.2 ± 7.9 × 10-3 1.3 × 101 ± 3.6 1.9 × 10-1 ± 3.2 × 10-3
W37 2.7 × 101 ± 3.8 2.7 × 10-2 ± 4.7 × 10-3 8.2 ± 4.6 1.1 × 10-1 ± 2.6 × 10-2
20 W33 1.6 × 101 ± 1.4 1.1 × 101 ± 1.2 1.2 × 101 ± 5.5 8.6 × 10-2 ± 1.5 × 10-2
W37 1.0 × 101 ± 6.4 × 10-2 1.1 × 101 ± 1.4 1.2 × 101 ± 4.7 1.1 × 10-1 ± 3.1 × 10-2
21 W33 5.6 × 101 ± 8.3 1.7 × 10-2 ± 1.7 × 10-3 2.1 × 101 ± 1.0 × 101 1.3 × 10-1 ± 2.0 × 10-2
W37 6.4 × 101 ± 1.5 3.3 × 10-2 ± 8.7 × 10-3 2.2 × 101 ± 1.0 × 101 1.2 × 10-1 ± 2.4 × 10-2
22 W33 4.3 × 101 ± 2.0 1.2 × 10-1 ± 2.8 × 10-2 2.3 × 10-1 ± 1.5 × 10-2 0.0 ± 0.0
W37 6.8 × 101 ± 5.1 2.7 × 10-2 ± 6.6 × 10-3 1.9 × 10-1 ± 2.0 × 10-2 0.0 ± 0.0
23 W33 2.6 × 101 ± 5.6 2.3 × 10-1 ± 3.6 × 10-2 0.0 ± 0.0 0.0 ± 0.0
W37 6.3 × 101 ± 2.0 8.2 × 10-3 ± 1.9 × 10-3 1.6 × 10-1 ± 2.9 × 10-2 5.3 × 10-1 ± 1.8 × 10-1
24 W33 1.2 × 101 ± 1.0 2.7 × 101 ± 2.1 × 10-1 1.8 ± 1.5 × 10-1 6.8 × 10-1 ± 3.4 × 10-2
W37 7.5 × 101 ± 3.8 9.7 × 10-3 ± 3.7 × 10-3 3.7 × 10-1 ± 3.4 × 10-2 0.0 ± 0.0
25 W33 6.5 × 101 ± 1.0 × 101 3.0 × 10-2 ± 1.0 × 10-2 7.5 × 10-2 ± 7.5 × 10-3 0.0 ± 0.0
W37 6.6 × 101 ± 7.1 9.1 × 10-3 ± 5.1 × 10-4 2.5 × 10-1 ± 2.7 × 10-2 0.0 ± 0.0
26 W33 8.5 × 101 ± 6.3 4.4 ± 9.3 × 10-1 3.2 × 10-1 ± 3.9 × 10-2 0.0 ± 0.0
W37 5.4 × 101 ± 4.5 2.0 × 10-2 ± 6.1 × 10-4 3.6 × 10-1 ± 4.2 × 10-2 0.0 ± 0.0
27 W33 7.0 × 101 ± 1.5 × 101 3.3 × 10-2 ± 4.7 × 10-3 2.8 × 10-1 ± 2.6 × 10-2 0.0 ± 0.0
W37 6.6 × 101 ± 3.6 × 10-1 2.1 × 10-2 ± 1.6 × 10-2 4.0 × 10-1 ± 3.8 × 10-2 0.0 ± 0.0

Vitali et al.

Vitali et al. BMC Microbiology 2012 12:236   doi:10.1186/1471-2180-12-236

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