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Open Access Research article

Genotyping of B. licheniformis based on a novel multi-locus sequence typing (MLST) scheme

Elisabeth H Madslien12, Jaran S Olsen1, Per E Granum2 and Janet M Blatny1*

Author Affiliations

1 Forsvarets Forskningsinstitutt FFI, Norwegian Defence Research Establishment, P. O. Box 25, Kjeller, N-2027, Norway

2 Department of Food Safety and Infection Biology, Section for Food Safety, Norwegian School of Veterinary Science, P. O. Box 8146 Dep, Oslo, N-0033, Norway

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BMC Microbiology 2012, 12:230  doi:10.1186/1471-2180-12-230

Published: 10 October 2012

Additional files

Additional file 1:

Cluster analysis of individual MLST candidate loci. Dendograms of each candidate-locus (adk, ccpA, glpT, gyrB, pyrE, recF, rpoB, spo0A and sucC) were drawn in MEGA4 using the NJ-method [57]. The quality of each branch is calculated using the bootstrap test with 500 replicates and are shown next to the branches [58]. Branch lengths were estimated using the Maximum Composite Likelihood Method [47].

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Additional file 2:

Table which shows strain identity, allels, sequence type (ST) and source of the 53 strains that were used in this study.

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Additional file 3:

Concatenated dendogram. The dendogram was constructed in MEGA5 [49] using the NJ-method on the concatenated sequences of the MLST loci (adk, ccpA, recF, rpoB, spo0A and sucC) [57] . The optimal tree with the sum of branch length 0.0487 is shown. The quality of each branch is calculated using the bootstrap test with 500 replicates and are shown next to the branches [58]. A total of 3189 positions were included in the dataset.

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