Table 1

Strains and plasmids used in this study

Strain/Plasmid

Characteristics *

Source


    STRAINS

E. coli TOP10

Invitrogen

L. lactis NZ9000

Plasmid-free strain

[37,38]

E. durans IPLA655

Isolated from artisanal cheese. Tyramine producer

IPLA Collection

    PLASMIDS

pUC18

AmpR

[39]

pNZ9530

EryR, nisR-nisK

[40]

pILORI4

EryR, lacZ promoterless gene

[41]

pNZcLIC

CmR, expression vector

[42]

pDA12

AmpR, pUC18 with PtyrS cloned in SmaI

This work

pDA15

AmpR, pUC18 with PtyrSΔ cloned in SmaI

This work

pDA16

EryR, pILORI4 including PtyrSΔ -lacZ fusion

This work

pNZcTyrS

CmR, pNZcLIC including tyrS

This work


* AmpR, ampicillin resistant; EryR, erythromycin resistant; CmR, chloramphenicol resistant

Linares et al. BMC Microbiology 2012 12:23   doi:10.1186/1471-2180-12-23

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