Table 2

Sequences, labels and formamide concentrations for FISH Probes
Organism Name Type Labels FA1 WB2 Sequence (5’ → 3’) References
A. oris L-Act476-2 LNA3 Cy3, FAM, 6-Rox 40% 46 mM ATCCAGCTACCGTCAACC [11]
C. rectus CAMP665 DNA Cy3, Cy5 30% 112 mM CATCTGCCTCTCCCTYAC [11]
F. nucleatum FUS664 Cy3, Cy5, FAM, FITC 40% 46 mM CTTGTAGTTCCGCYTACCTC [32]
Fnuc133c DNA Cy3, Cy5 40% 46 mM GTTGTCCCTANCTGTGAGGC [11]
P. intermedia L-Pint649-2 LNA Cy3, FAM,6-Rox 40% 46 mM CGTTGCGTGCACTCAAGTC [11]
P. gingivalis L-Pgin1006-2 LNA3 Cy3, Cy5, FAM 30% 112 mM GTTTTCACCATCMGTCATC [11]
Streptococci STR405 DNA Cy3, Cy5 20% 215 mM TAGCCGTCCCTTTCTGGT [34]
T. denticola TrepG1_679 DNA Cy3, Cy5, FAM 40% 46 mM GATTCCACCCCTACACTT [13]
T. forsythia Tfor997 DNA Cy3, Cy5, FAM 40% 46 mM TCACTCTCCGTCGTCTAC [35]
V. dispar VEI217 DNA Cy3, Cy5, FAM, FITC, 6-Rox 40% 46 mM AATCCCCTCCTTCAGTGA [32]

1 Formamide concentration in the hybridisation buffer.

2 Concentration of NaCl used in the washing buffer.

3 Probes containing locked nucleic acid substitutes (LNA). The ribose ring of LNA is constrained by a methylene linkage between the 2’ oxygen and the 4’ carbon.

Ammann et al.

Ammann et al. BMC Microbiology 2012 12:227   doi:10.1186/1471-2180-12-227

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