Table 2

Culture conditions of D. hafniense DCB-2

Experiments

Basal medium

Carbon/e- donor

e- acceptor/substrate added

Headspace gas

Comments


Pyruvate fermentation

DCB1*, vitamins

Pyruvate, 20 mM

N2, 95%

CO2, 5%

Reference culture for microarray


Fe(III) reduction

CBF**, vitamins

Lactate, 20 mM

Ferric citrate, 50 mM or

Ferric oxide, 50 mM

N2, 95%

CO2, 5%

Ferric citrate for microarray

Ferric oxide for growth study only


Se(VI) reduction

DCB1, vitamins

Pyruvate, 20 mM

Sodium selenate, 1 mM

N2, 95%

CO2, 5%

For microarray


U(VI) reduction

DCB1, vitamins

Pyruvate, 20 mM

Uranyl acetate, 0.5 mM

N2, 95%

CO2, 5%

For microarray


As(V) reduction

DCB1, vitamins

Pyruvate, 20 mM

Sodium arsenate, 1 mM

N2, 95%

CO2, 5%

For growth study only


Nitrate reduction

CBF, vitamins

Lactate, 20 mM

Potassium nitrate, 10 mM

N2, 95%

CO2, 5%

For microarray


DMSO/TMAO

reduction

DCB1, vitamins

Lactate, 20 mM

DMSO, 5 mM or

TMAO, 5 mM

N2, 95%

CO2, 5%

For growth study only


3-Cl-4-OH-BA dechlorination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

3-chloro-4-hydroxybenzoate

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study


3,5-DCP dechlorination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

3,5-dichlorophenol

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study


o-BP debromination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

ortho-bromophenol

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study


Oxygen effect

DCB1, vitamins

Pyruvate, 20 mM

N2, 95%

CO2, 5%

Exposure to air for 3 hours after fermentative cell growth


N2 fixation

DCB1, vitamins

Pyruvate, 20 mM

N2, 95%

CO2, 5%

NH4+ omitted from DCB1

Gas replenished every 12 h


CO2 fixation

DCB1, vitamins

CO, CO2

N2, H2

Details in Figure 3


DCB1*, modified DCB1 medium [61]

CBF**, modified CBF medium [32]

Kim et al. BMC Microbiology 2012 12:21   doi:10.1186/1471-2180-12-21

Open Data