Table 1

Bacterial strains and plasmids used in this study
Strain or plasmid Relevant genotype and/or description Source or reference
R. etlistrains
 CFN 42 Wild type [29]
 CE3 Spontaneous Smr mutant of R, etli CFN 42 Nalr [31]
 CMS310 R. etli CE3 otsAch::ΩNalrSmrSpcr This study
E. colistrain
 DH5α supE44Δ (lac)U169 ϕ80d lacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1; host for DNA manipulations [35]
Plasmids
 pSK(-) Cloning vector; Apr Stratagene
 pUC19-301 Cloning vector, Apr J. Onofre, Personal Communication
 pHP45Ω pBR322 derivative carrying the Ω cassette; AprSmrSpr [36]
 pRK600 Helper plasmid; Cmrtra [37]
 pJQ200-SK Suicide vector; Gmrmobsac [38]
 pMotsA1 4.2-kb blunt fragment from R. etli CE3 genome (containing frk, otsAch, pgi) cloned into pUC19301 in EcoRV; Apr This study
 pMotsA4 4,1-kb BglII-XbaI fragment from pMotsA1 cloned into pSK in BamHI-XbaI; Apr This study
 pMotsA5 pMotsA4 derivative containing an BglII recognition site within otsAch; Apr This study
 pMotsA6 pMotsA5 derivative with Ω casete within otsAch; AprSmrSpcr This study
 pMotsA7 6.1-kb ApaI-XbaI fragment from pMotsA6 (containing frk,otsAch, pgi) cloned into pJQ200-SK; GmrSmrSpcr This study

Reina-Bueno et al.

Reina-Bueno et al. BMC Microbiology 2012 12:207   doi:10.1186/1471-2180-12-207

Open Data