Table 1 |
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Identification of metabolites formed during transformation of different CNACs by strain SJ98 |
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|
GC Rt of substrates and metabolites (min) |
HPLC Rt of substrates and metabolites (min) |
Identified metabolites |
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|
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|
Substrate |
Metabolite |
Substrate |
Metabolite |
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|
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Test compounds |
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|
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|
2C4NP |
2.66 |
2.43, 4.18, 5.99 |
2.16 |
1.98, 3.58, 4.21 |
PNP, 4NC, BT |
|
|
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|
2C3NP |
2.42 |
2.31 |
2.07 |
1.86,3.49 |
MNP, 3NC |
|
|
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|
4C2NP |
2.24 |
ND |
2.03 |
ND |
ND |
|
|
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|
2C4NB |
2.74 |
2.1, 3.60 |
19.45 |
3.53 |
PNB, 3,4DHBA |
|
|
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|
4C2NB |
2.51 |
2.88, 3.26 |
21.87 |
2.36, 3.89 |
ONB, 3HAA |
|
|
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|
5C2NB |
2.52 |
2.875, 3.24 |
26.98 |
2.41, 3.92 |
ONB, 3HAA |
|
|
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|
Standards |
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|
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|
PNP |
2.44 |
1.99 |
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|
|
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|
4NC |
4.17 |
3.59 |
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|
|
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|
BT |
5.94 |
4.19 |
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|
|
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|
MNP |
2.32 |
1.88 |
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|
|
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|
3-Nitrocatechol |
ND |
3.50 |
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|
|
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|
PNB |
2.11 |
3.53 |
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|
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|
3,4DHBA |
3.60 |
ND |
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|
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|
ONB |
2.88 |
2.36 |
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|
|
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|
3HAA |
3.25 |
3.91 |
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|
|
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|
ND not determined; PNP p-nitrophenol, 4NC 4-nitrocatechol, BT benzenetriol, MNP m-nitrophenol, 3NC 3-nitrocatechol, PNB p-nitrobenzoate, 3,4DHBA 3,4- dihydrooxybenzoate, ONB o-nitrobenzoate, 3HAA 3-hydroxyanthranilic acid |
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|
Pandey et al. BMC Microbiology 2012 12:19 doi:10.1186/1471-2180-12-19 |
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