Table 1

Identification of metabolites formed during transformation of different CNACs by strain SJ98

GC Rt of substrates and metabolites (min)

HPLC Rt of substrates and metabolites (min)

Identified metabolites


Substrate

Metabolite

Substrate

Metabolite


Test compounds


2C4NP

2.66

2.43, 4.18, 5.99

2.16

1.98, 3.58, 4.21

PNP, 4NC, BT


2C3NP

2.42

2.31

2.07

1.86,3.49

MNP, 3NC


4C2NP

2.24

ND

2.03

ND

ND


2C4NB

2.74

2.1, 3.60

19.45

3.53

PNB, 3,4DHBA


4C2NB

2.51

2.88, 3.26

21.87

2.36, 3.89

ONB, 3HAA


5C2NB

2.52

2.875, 3.24

26.98

2.41, 3.92

ONB, 3HAA


Standards


PNP

2.44

1.99


4NC

4.17

3.59


BT

5.94

4.19


MNP

2.32

1.88


3-Nitrocatechol

ND

3.50


PNB

2.11

3.53


3,4DHBA

3.60

ND


ONB

2.88

2.36


3HAA

3.25

3.91


ND not determined; PNP p-nitrophenol, 4NC 4-nitrocatechol, BT benzenetriol, MNP m-nitrophenol, 3NC 3-nitrocatechol, PNB p-nitrobenzoate, 3,4DHBA 3,4- dihydrooxybenzoate, ONB o-nitrobenzoate, 3HAA 3-hydroxyanthranilic acid

Pandey et al. BMC Microbiology 2012 12:19   doi:10.1186/1471-2180-12-19

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