Table 1

Genus specific 16S rRNA targeted bacterial primers used in this study
Sr no. Genus Primer sequence PCR Product (bp) Tm(ºC) References
1. Methanobrevibactr F 5’- CGATGCGGACTTGGTGTTG-3’ 184 59.7 [21]
R 5’-TGTCGCCTCTGGTGAGATGTC-3’ 59.8
2. Peptostreptococcus F 5’-AACTCCGGTGGTATCAGATG-3’ 270 55.4 [1]
R 5’-GGGGCTTCTGAGTCAGGTA-3’ 56.4
3. Ruminococcus F 5’-GAAAGCGTGGGGAGCAAACAGG-3’ 302 65.8 [21]
R 5’- GACGACAACCATGCACCACCTG-3’ 64.4
4. Eubacterium F 5’-GTAGTCCACGCCGTAAACGATG-3’ 278 60.4 [21]
R 5’-ACACGAGCTGACGACAACCATG-3’ 62.4
5. Bacteroides F 5’- GGGGTTCTGAGAGGAAG-3’ 115 54.0 [21]
R 5’- GCTACTTGGCTGGTTCAG-3’ 56.0
6. Lactobacillus F 5’-GCAGCAGTAGGGAATCTTCCA-3’ 340 64.0 [25]
R 5’-GCATTYCACCGCTACACATG-3’ 58.0
7. Clostridium leptum subgroup F 5’-CGTCAGCTCGTGTCGTGAGAT-3’ 125 60.0 [21]
R 5’-CGTCATCCCCACCTTCCTCC-3’ 62.5
8. Clostridium coccoides subgroup F 5’-GCCACATTGGGACTGAGA-3’ 170 56.0 This study
R 5’-GCTTCTTAGTCAGGTACCG-3’ 58.0
9. Campylobacter F 5’-AGGGAATATTGCGCAATGGGGGAAA-3’ 180 58.0 [21]
R 5’- GATTCCGAGTAACGCTTGCACCCT-3’ 59.0
10. Bifidobacterium F 5’-GATTCTGGCTCAGGATGAACGC-3’ 231 61.9 [21]
R 5’-CTGATAGGACGCGACCCCAT-3’ 60.8
11. Sulfate-reducing bacteria (APS reductase subunit A gene) F 5’-TGGCAGATMATGATYMACGG-3’ 396 54. This study
R 5’-GGCCGTAACCGTCCTTGAA-3’ 54.0

Verma et al.

Verma et al. BMC Microbiology 2012 12:183   doi:10.1186/1471-2180-12-183

Open Data